2016 Fiscal Year Final Research Report
Functional analysis of lipo genes, PHGPx depleted novel cell death execution factors, identified by genome-wide shRNA library
| Project/Area Number |
26460075
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | Kitasato University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 細胞死 / 脂質酸化 / 遊離二価鉄 / shRNAライブラリー / フェロトーシス / GPx４欠損細胞死 / リポキシトーシス / ビタミンE |
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| Outline of Final Research Achievements |
To clarify the mechanism of PHGPx depleted novel cell death, we identified six PHGPx depleted lipid peroxidation dependent cell death (lipoxytosis) inducing genes (Lipo gene) by genome-wide shRNA library screening and cell death recovery screening by retransfection of cDNA for lipo gene into lipo gene knockdown cells. Knockdown cells of the Lipo gene failed to suppress ferroptosis by the anti-cancer agent erastin and RSL3. These results reveal that PHGPx-depleted cell death is different cell death pathway from ferroptosis by erastin and RSL3.
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| Free Research Field |
医歯薬学 薬学 生物系薬学
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