2017 Fiscal Year Final Research Report
Involvements of Cav1.3 in the generation of a sustained inward Na+ current in sinoatrial node cells
| Project/Area Number |
26460295
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
General physiology
|
|---|
| Research Institution | Shiga University of Medical Science |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
松浦 博 滋賀医科大学, 医学部, 教授 (60238962)
林 維光 滋賀医科大学, 医学部, 助教 (80242973)
|
|---|
| Project Period (FY) |
2014-04-01 – 2018-03-31
|
| Keywords | 心臓 / ペースメーカー活動 / イオンチャネル |
|---|
| Outline of Final Research Achievements |
The sustained inward Na+ current has been recorded in sinoatrial node cells and put foreword as a potential pacemaker current in the heart. However, molecular determinants for Ist remain unknown. The present study has investigated the involvement of Cav1.3 L-type Ca2+ channels in the generation of Ist. We found that genetic ablation of Cav1.3 resulted in total abolition of Ist. Then we applied a next-generation sequencing approach to examine potential splicing and RNA editing of Cav1.3 in sinoatrial node, but failed to provide evidence for the presence of variants that can produce a Na current. These results raise the possibility that Cav1.3 is functionally coupled to unknown Na-permable pathway to mediate Ist in cardiac pacemaker cells.
|
| Free Research Field |
生理学一般
|
