Sex differences in the GABAergic system

2016 Fiscal Year Final Research Report

• Project/Area Number: 26460314
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Environmental physiology(including physical medicine and nutritional physiology)
• Research Institution: Hirosaki University
• Principal Investigator: Yamada Junko 弘前大学, 保健学研究科, 教授 (30334965)
• Co-Investigator(Renkei-kenkyūsha): MIGITA Keisuke 福岡大学, 薬学部, 准教授 (10352262)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: GABA / 脳の性差 / トニック抑制

Outline of Final Research Achievements

In this study, we focused on GABAergic system to investigate the sex differences of the brain, using the behavior analysis, electrophysiology analysis and expression analysis of the GABAA receptor. The ovariectomized (OVX) wild type (WT) mice were more active than male mice in the open field test with a time course. The male PRIP-1 KO mice were more active than WT mice as the female mice.
To investigate the function of synaptic and extrasynaptic GABAA receptors, we utilized a subcelullar fractionation approach coupled with detergent extraction to prepare synaptic and extrasynaptic GABAA receptors from amygdala. The Pre-Post-Extra-synaptic membrane were prepared using a modified version of a previously method (Goebel-Goody et al, 2009, Garcão et al, 2014). This modified method makes us possible to extract protein from small amount of tissue (about 15-20 μg of protein of pre-synaptic membrane from 20 mg tissue).

Free Research Field

神経生理学