2016 Fiscal Year Final Research Report
Evaluation of potential clinical application of tight junction proteins in cervical adenocarcinoma
| Project/Area Number |
26460421
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Human pathology
|
|---|
| Research Institution | Sapporo Medical University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
SAWADA NORIMASA 札幌医科大学, 医学部, 教授 (30154149)
TANAKA SATOSHI 札幌医科大学, 医学部, 講師 (30374250)
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Keywords | 子宮頚部 / 子宮頚部腺癌 / タイト結合 / 治療・診断マーカー |
|---|
| Outline of Final Research Achievements |
We exam the expression of tight junction proteins in uterine cervical adenocarcinoma specimens. We found that expression of Claudin-1 and JAM-A was significantly higher in cervical adenocarcinoma than in non-neoplastic glands. In adenocarcinoma, localization of Claudin-1 and JAM-A was extended throughout the whole cell membranes, whereas they were predominantly expressed at the most apical cell-cell junction in non-neoplastic glands. Immunoreactivities and localization of Claudin-1 and JAM-A successfully distinguished neoplasms from non-neoplastic cervical glands with high specificity and high sensitivity. In cervical adenocarcinoma cell lines, gene knockout of Claudin-1 suppressed tumorigenicity, invasive capacity and tumor-initiation of cancer cells. Expression of Claudin-1 was mediated by ERK signaling. Targeting of Claudin-1 may be effective for cervical adenocarcinoma diagnosis and therapy.
|
| Free Research Field |
医歯薬学
|
