2016 Fiscal Year Final Research Report
Investigation of tumor microenvironment and signaling cascade of monocyte differentiation into macrophages.
| Project/Area Number |
26460422
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | Yokohama City University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Keywords | がん関連遺伝子 / 染色体 / コピー数 / 前がん病変 |
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| Outline of Final Research Achievements |
We investigated genotype-phenotype correlation in rare tumors, mainly those with FLCN germline mutations, using surgically resected specimens.
Approximately 40% of tumors with FLCN germline mutations had second hits or loss of heterozygosities. Those without second hits also demonstrated suppressed expression of FLCN at protein level, indicating that mutant FLCN had dominant-negative effects.
We successfully established some primary culture tumor cells from surgically resected renal tumors harboring FLCN germline mutations. We also established one immortalized cells using SV40T antigen.
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| Free Research Field |
人体病理
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