Hepatic stem cells transplantation activates the growth of intrinsic hepatic progenitor cells in recipient rat livers.

2016 Fiscal Year Final Research Report

• Project/Area Number: 26460474
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: Sapporo Medical University
• Principal Investigator: Ichinohe Norihisa 札幌医科大学, 医学部, 研究員 (80452978)
• Co-Investigator(Renkei-kenkyūsha): Mitaka Toshihiro 札幌医科大学, 医学部, 教授 (50231618) ; Tanimizu Naoki 札幌医科大学, 医学部, 准教授 (00333386)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 細胞・組織 / 再生医学 / 肝幹・前駆細胞 / 細胞移植 / 生体組織工学

Outline of Final Research Achievements

In retrorsine (Ret)/70% partial hepatectomy (PH) model rat livers small hepatocyte-like progenitor cells (SHPCs) transiently appear to form clusters. However, the molecular mechanisms regulating their proliferation remain unknown. We found that transplantation of Thy1+ cells isolated from galactosamine-treated rat livers transiently increased the liver mass by expanding SHPCs in Ret/PH model livers. In this study, we examined the mechanism of the enhanced liver regeneration. Extracellular vesicles secreted from Thy1+ cells induced interleukin 17 b (IL17b) and IL25 expression in sinusoidal endothelial cells and Kupffer cells, respectively, whereas they induced IL17 receptor b (IL17rb) in SHPCs. In conclusion, Thy1+ cells transplantation enhanced the growth of intrinsic hepatic progenitor cells via IL17RB signals.

Free Research Field

医歯薬学