Proteome analysis of toxoplasmic retinochoroiditis-a quest of biomarker

2016 Fiscal Year Final Research Report

• Project/Area Number: 26460505
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Parasitology (including sanitary zoology)
• Research Institution: Chiba University
• Principal Investigator: Norose Kazumi 千葉大学, 大学院医学研究院, 准教授 (30156244)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: トキソプラズマ / トキソプラズマ性網脈絡膜炎 / プロテオーム解析 / バイマーカー

Outline of Final Research Achievements

We established successfully a murine model of ocular toxoplasmosis (OT) infected perorally. Observations with fundus camera and fluorescein angiogram revealed that this model resembled with the human OT. The levels of cytokines and chemokines in aqueous humor, cerebrospinal fluid and serum were investigated and some molecules were identified to increase significantly after infection. In addition, some specific proteins were identified to either increase or decrease 2-fold after infection by the analysis on mouse retina using two-dimensional electrophoresis and mass spectrometry. It is suggestive that these molecules may play pivotal roles in the pathogenesis of OT and that analyzing these molecules is helpful to diagnose human OT during its early stage.

Free Research Field

寄生虫学