Functional analysis of B. bronchiseptica gene products specifically induced in rat trachea

2016 Fiscal Year Final Research Report

• Project/Area Number: 26460525
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bacteriology (including mycology)
• Research Institution: Osaka University
• Principal Investigator: Abe Hiroyuki 大阪大学, 微生物病研究所, 助教 (00379265)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 病原生遺伝子 / 百日咳菌 / 気管支敗血症菌

Outline of Final Research Achievements

Bordetella bronchiseptica, a Gram-negative pathogenic bacterium causing respiratory infections in a broad range of mammals, is considered to have an evolutionary progenitor-like relationship to Bordetella pertussis and Bordetella parapertussis, which are causative agents of pertussis in humans. Previous comparative genomic analysis revealed that genetic differences may be associated with adaptation to the different hosts and different manifestations of disease. To get a clue to understand the underlying genetic differences associated with broad host range of B. bronchiseptica, we constructed several deletion mutant of genes specifically induced in rat trachea or with long-range deletion of B. bronchiseptica specific gene loci and examined whether these loci are involved in bacterial colonization of rat trachea. However, no apparent difference was found between wild-type B. bronchiseptica and mutants in its ability to establish respiratory infection in rats.

Free Research Field

病原細菌学