Elucidation of caspase-1 activation inhivition mechanism by Vibrio parahaemolyticus effector VopQ

2016 Fiscal Year Final Research Report

• Project/Area Number: 26460531
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bacteriology (including mycology)
• Research Institution: University of the Ryukyus
• Principal Investigator: HIGA Naomi 琉球大学, 医学(系)研究科(研究院), 技術専門職員 (70457688)
• Co-Investigator(Renkei-kenkyūsha): SUZUKI Toshihiko 東京医科歯科大学, 医歯学総合研究科, 教授 (10292848)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: マクロファージ / インフラマゾーム / 感染防御

Outline of Final Research Achievements

The effector protein VopQ of Vibrio parahaemolyticus is known to inhibit the activation of caspase-1 in macrophage inflammasome. I attempted to clarify, the relationship between autophagy induction and inflammasome activation inhibition, searching for target molecules for VopQ in host cells. As a result, the cell adhesion factor Afadin was a candidate the target molecule for VopQ, but interaction in macrophages was not observed. In addition, I could not identify such concerning the relation with autophagy, during this reserch period, but ATG5 conditional knockout mouse expressing specifically on phagocytic cells was made, so it can be expected to be utilized in future research.

Free Research Field

細菌学