The mechanism by which influenza C virus NS1 protein regulates virus replication

2016 Fiscal Year Final Research Report

• Project/Area Number: 26460547
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Virology
• Research Institution: Yamagata University
• Principal Investigator: Hongo Seiji 山形大学, 医学部, 教授 (90229245)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: C型インフルエンザ / ウイルス / NS1 / 核移行シグナル / 核外移行シグナル

Outline of Final Research Achievements

Influenza C virus NS1 protein localized in the nucleus and cytoplasm in the early and late phase of infection, respectively, suggested that the NS1 protein may possess a nuclear localization signal (NLS) and a nuclear export signal (NES). We analyzed the deduced amino acid sequences of the NS1 and found that two overlapping leucine-rich NES-like sequences are present at residues 109-126, and that four basic amino acid stretches are present at residues 36-46, 87-90, 167-171, and 189-196 as the candidates for NLSs. The nuclear export of the NS1 mutant which lost residues 109-126 was severely impaired, suggesting that NES is present at residues 109-126. The nuclear import of the NS1 mutant which lost residues 167-171 or 189-196 was severely impaired, suggesting that NLS is present at residues 167-171 and 189-196.

Free Research Field

医歯薬学