Analysis of regulation mechanisms of hepatitis B virus life cycle by kinases and post-translational modifications

2017 Fiscal Year Annual Research Report

• Project/Area Number: 26460565
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: 渡士 幸一 国立感染症研究所, ウイルス第二部, 主任研究官 (40378948)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: HBV

Outline of Annual Research Achievements

B型肝炎ウイルス(HBV)複製培養系において、細胞骨格のうち微小管がHBV coreタンパク質と相互作用し、HBV coreタンパク質の多量体化によるキャプシド形成に関わっていることが示された。この制御機構は、微小管形成阻害剤であるノコダゾールやビンブラスチン、コルヒチンを処理することにより阻害され、結果的に細胞内のHBVキャプシド形成量が低下し、ウイルス複製活性が減少することが認められた。この結果は細胞をコンフルエントの状態で得られたものであり、細胞毒性も認められなかったことから、微小管への効果に依存すると考えられた。これまで我々はテトラサイクリンによりHBV複製をon/off可能なHepAD38細胞を親株として、シングルセルクローニング法によってHBV複製活性の高い細胞クローン（Hep38.7-Tet細胞）と低い細胞クローン（Hep38.3-Tet細胞）を得ていたが、後者に比較して前者では微小管タンパク質チューブリンとHBV coreタンパク質の相互作用が強く、またキャプシド形成活性が高いことが明らかとなった。このことより、今回明らかにした機構は実際のHBV複製活性制御に重要であることが示唆された。これは査読付き国際誌に論文として発表した。また一方で、チューブリンの翻訳後修飾がHBV複製活性を制御していることを示唆するデータが得られた。このことから、キナーゼなどを含めた因子と微小管の関連を解析することにより、今後新たなHBV複製機構、また創薬標的が明らかになると期待される。

Research Products

• [Int'l Joint Research] Los Alamos National Laboratory/Indiana University School of Medicine(米国)
  • Country Name: U.S.A.
  • Counterpart Institution: Los Alamos National Laboratory/Indiana University School of Medicine
• [Int'l Joint Research] University of the Witwatersrand(南アフリカ)
  • Country Name: SOUTH AFRICA
  • Counterpart Institution: University of the Witwatersrand
• [Int'l Joint Research] INTS(フランス)
  • Country Name: FRANCE
  • Counterpart Institution: INTS
• [Journal Article] Novel stable HBV producing cell line systems for expression and screening antiviral inhibitor of hepatitis B virus in human hepatoma cell line (2018)
  • Author(s): Ogura N, Ogawa K, Watashi K, Ito T, Wakita T
  • Journal Title: Biochem Biophys Res Commun Volume: 498 Pages: 64-71
  • DOI: 10.1016/j.bbrc.2018.02.175
  • Peer Reviewed
• [Journal Article] De Novo Macrocyclic Peptide Inhibitors of Hepatitis B Virus Cellular Entry (2018)
  • Author(s): Passioura T, Watashi K, Fukano K, Shimura S, Saso W, Morishita R, Ogasawara Y, Tanaka Y, Mizokami M, Sureau C, Suga H, Wakita T
  • Journal Title: Cell Chem Biol Volume: S2451-9456(18) Pages: 30125-30129
  • DOI: 10.1016/j.chembiol.2018.04.011
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] 冠Award受賞者の声 平成29年度第16回MSD Award (2018)
  • Author(s): 渡士幸一
  • Journal Title: 肝臓 Volume: 59 Pages: 71
• [Journal Article] Hepatitis C virus entry inhibitors for optimally boosting direct-acting antiviral-based treatments (2017)
  • Author(s): Ohashi H, Koizumi Y, Fukano K, Wakita T, Perelson AS, Iwami S, Watashi K
  • Journal Title: Proc Natl Acad Sci U S A Volume: 114 Pages: E4527-E4529
  • DOI: 10.1073/pnas.1705234114
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Hepatitis B virus prevents excessive viral production via reduction of cell death-inducing DFF45-like effectors (2017)
  • Author(s): Yasumoto J, Kasai H, Yoshimura K, Otoguro T, Watashi K, Wakita T, Yamashita A, Tanaka T, Takeda S, Moriishi K
  • Journal Title: J Gen Virol Volume: 98 Pages: 1762-1773
  • DOI: 10.1099/jgv.0.000813
  • Peer Reviewed
• [Journal Article] ssAAVs containing cassettes encoding SaCas9 and guides targeting hepatitis B virus inactivate replication of the virus in cultured cells (2017)
  • Author(s): Scott T, Moyo B, Nicholson S, Maepa MB, Watashi K, Ely A, Weinberg MS, Arbuthnot P
  • Journal Title: Sci Rep Volume: 7 Pages: 7401
  • DOI: 10.1038/s41598-017-07642-6
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Functional association of cellular microtubules with viral capsid assembly supports efficient hepatitis B virus replication (2017)
  • Author(s): Iwamoto M, Cai D, Sugiyama M, Suzuki R, Aizaki H, Ryo A, Ohtani N, Tanaka Y, Mizokami M, Wakita T, Guo H, Watashi K
  • Journal Title: Sci Rep Volume: 7 Pages: 10620
  • DOI: 10.1038/s41598-017-11015-4
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Involvement of PUF60 in Transcriptional and Post-transcriptional Regulation of Hepatitis B Virus Pregenomic RNA Expression (2017)
  • Author(s): Sun S, Nakashima K, Ito M, Li Y, Chida T, Takahashi H, Watashi K, Sawasaki T, Wakita T, Suzuki T
  • Journal Title: Sci Rep Volume: 7 Pages: 12874
  • DOI: 10.1038/s41598-017-12497-y
  • Peer Reviewed / Open Access
• [Journal Article] Chemical array system, a platform to identify novel hepatitis B virus entry inhibitors targeting sodium taurocholate cotransporting polypeptide (2017)
  • Author(s): Kaneko M, Futamura Y, Tsukuda S, Kondoh Y, Sekine T, Hirano H, Fukano K, Ohashi H, Saso W, Morishita R, Matsunaga S, Kawai F, Ryo A, Park SY, Suzuki R, Aizaki H, Ohtani N, Sureau C, Wakita T, Osada H, Watashi K
  • Journal Title: Sci Rep Volume: 8 Pages: 2769
  • DOI: 10.1038/s41598-018-20987-w
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 抗B型肝炎ウイルス薬の現状と新規薬剤開発のアプローチ (2017)
  • Author(s): 九十田千子、渡士幸一
  • Journal Title: 化学療法の領域 Volume: 33 Pages: 73-78
• [Journal Article] 抗ウイルス薬の個性を知る ～ウイルス学と数学の融合研究によるC型肝炎治療薬併用の薬効評価と最適化～ (2017)
  • Author(s): 大橋啓史、渡士幸一
  • Journal Title: ウイルス Volume: 67 Pages: 133-142
• [Journal Article] 目覚ましいC型肝炎治療の進歩 (2017)
  • Author(s): 渡士幸一
  • Journal Title: 感染 炎症 免疫 Volume: 47 Pages: 71-72
• [Presentation] B型肝炎ウイルスの培養系と創薬研究 (2017)
  • Author(s): 渡士幸一
  • Organizer: 第１９回 仙台 Liver Meeting
  • Invited
• [Presentation] Ｂ型肝炎ウイルス基礎研究・創薬研究の最近の知見 (2017)
  • Author(s): 渡士幸一
  • Organizer: 衛生微生物技術協議会第38回研究会
  • Invited
• [Presentation] トランスポーターと肝炎ウイルス (2017)
  • Author(s): 渡士幸一
  • Organizer: 日本薬物動態学会第32回年会
  • Invited
• [Presentation] B型肝炎ウイルス培養系の構築から創薬研究へ (2017)
  • Author(s): 渡士幸一
  • Organizer: 日本薬学会第138年会
  • Invited
• [Presentation] Targeting HBV-X by regulating Non-Stop mediated RNA decay (2017)
  • Author(s): Aly HH, Shiromoto F, Kato T, Watashi K, Chayama K, Wakita T
  • Organizer: 5th JAPAN-TAIWAN-KOREA HBV Research Symposium 2017
  • Int'l Joint Research
• [Presentation] Acute Hepatitis B in Japan, April 1999 ~ December 2015 (2017)
  • Author(s): Zheng X, Aoyagi H, Hossam G, Zaitsu T, Puig-Basagoiti F, Kao Y, Watashi K, Suzuki R, Takahashi T, Sunagawa T, Oishi K, Wakita T, Aizaki H
  • Organizer: 5th JAPAN-TAIWAN-KOREA HBV Research Symposium 2017
  • Int'l Joint Research
• [Presentation] Identification of HBV entry inhibitors without interfering with the NTCP transporter activity (2017)
  • Author(s): Fukano K, Shimura S, Peel M, Sluder A, Kawai F, Tsukuda S, Suzuki R, Aizaki H, Park SY, Wakita T, Ogasawara Y, Watashi K
  • Organizer: 5th JAPAN-TAIWAN-KOREA HBV Research Symposium 2017
  • Int'l Joint Research
• [Presentation] 抗HBV効果を有するNeplanocin A誘導体の作用機序解析 (2017)
  • Author(s): 外山政明, 濱崎隆之, 岡本実佳, 渡士幸一, 脇田隆字, Ashoke Sharon, 馬場昌範
  • Organizer: 第27回抗ウイルス療法学会学術集会・総会
• [Presentation] 抗肝炎ウイルス薬の薬効・変異頻度定量評価系の開発と多剤併用の意義 (2017)
  • Author(s): 渡士幸一, 小泉吉輝, 大橋啓史, 中嶋翔, 田中靖人, 脇田隆字, Alan S. Perelson, 岩見真吾
  • Organizer: 第27回抗ウイルス療法学会学術集会・総会
• [Presentation] C型慢性肝炎のウイルス学的著効（SVR）後の肝実質細胞のオルガネラ異常の解析 (2017)
  • Author(s): 青柳東代, 飯島尋子, Francesc Puig-Basagoiti, Zheng Xin, Yu Ting Kao, Gewaid E. Hossam, 在津拓馬, 松田麻未, 渡士幸一, 鈴木亮介, 政木隆博, 島田紀明, 加藤慶三, 坪田昭人, 三又絢子, 酒巻有里子, 市野瀬志津子, 和氣健二郎, 脇田隆字, 相崎英樹
  • Organizer: 第27回抗ウイルス療法学会学術集会・総会
• [Presentation] B型肝炎ウイルス培養系を用いた創薬研究 (2017)
  • Author(s): 渡士幸一、九十田千子、脇田隆字
  • Organizer: 第53回日本肝臓学会総会
• [Presentation] 胆汁酸代謝調節機構を標的としたB型肝炎ウイルス制御の試み (2017)
  • Author(s): 伊藤清顕、渡士幸一、米田政志
  • Organizer: 第53回日本肝臓学会総会
• [Presentation] HCVの肝星細胞への感染および肝星細胞活性化のメカニズム解析 (2017)
  • Author(s): 相崎英樹、渡士幸一、鈴木哲朗、政木隆博、横山寛、脇田隆字
  • Organizer: 第53回日本肝臓学会総会
• [Presentation] 1999年から2015年までの日本におけるB型急性肝炎4273症例の疫学解析 (2017)
  • Author(s): Zheng Xin, 青柳東代, Gewaid E. Hossam, 在津拓馬, Puig-Basagoiti Francesc, Yu Ting Kao, 渡士幸一, 鈴木亮介, 高橋琢理, 砂川富正, 大石和徳, 脇田隆字. 相崎英樹
  • Organizer: 第53回日本肝臓学会総会
• [Presentation] Abnormal organelles were continuously observed in sustained virological response (SVR) patients (2017)
  • Author(s): Aoyagi H, Iijima H, Puig-Basagoiti F, Zheng X, Kao YT, Gewaid HE, Zaitsu T, Matsuda M, Watashi K, Suzuki R, Masaki T, Shimada N, Kato K, Tsubota A, Mimata A, Sakamaki Y, Ichinose S, Wake K, Wakita T, Aizaki H
  • Organizer: The Asian Pacific Associaton for the Study of the Liver - Single Topic Conference 2017
  • Int'l Joint Research
• [Presentation] Acute hepatitis C virus infection among HIV-infected men who have sex with men (2017)
  • Author(s): Aoyagi H, Itoda I, Zheng X, Gewaid HE, Puig-Basagoiti F, Kao YT, Watashi K, Suzuki R, Wakita T, Aizaki H
  • Organizer: APASL Single Topic Conference 2017 Mongolia 6th HCV Conference on HCV and co-infections
  • Int'l Joint Research
• [Presentation] Sphingomyelin participates in the formation of Hepatitis C virus membrane replication vesicles (2017)
  • Author(s): Gewaid HE, Aoyagi H, Arita M, Watashi K, Suzuki R, Aly HH, Kumagai K, Yamaji T, Fukasawa M, Mimata A, Sakamaki Y, Ichinose S, Hanada K, Wakita T, Aizaki H
  • Organizer: American Society for Virology - 2017 Annual Meeting
  • Int'l Joint Research
• [Presentation] The integrity of cellular microtubule structure is important for an efficient HBV capsid formation (2017)
  • Author(s): Iwamoto M, Cai D, Sugiyama M, Suzuki R, Aizaki H, Ryo A, Ohtani N, Tanaka Y, Mizokami M, Wakita T, Guo H, Watashi K
  • Organizer: 2017 International HBV Meeting The Molecular Biology of Hepatitis B Viruses
  • Int'l Joint Research
• [Presentation] Supporting evidence that HBV virions bud mainly through the ER/ERGIC membranes (2017)
  • Author(s): Hotta H, Chen M, Aoki-Utsubo C, Deng L, Watashi K, Wakita T
  • Organizer: 2017 International HBV Meeting The Molecular Biology of Hepatitis B Viruses
  • Int'l Joint Research
• [Presentation] HBV-X and the balance between degradation or inflammation (2017)
  • Author(s): Aly HH, Shiromoto F, Watashi K, Hoshino S, Kato T, Chayama K, Wakita T
  • Organizer: 2017 International HBV Meeting The Molecular Biology of Hepatitis B Viruses
  • Int'l Joint Research
• [Presentation] SLC10A1/NTCPのトラフィッキング制御を標的とした新規B型肝炎ウイルス感染阻害戦略 (2017)
  • Author(s): 深野顕人, 九十田千子, 鈴木亮介, 相崎英樹, 村松正道, 脇田隆字, 小笠原裕樹, 渡士幸一
  • Organizer: 日本薬学会第138年会
• [Presentation] HBV exploit Ski2/RNA complex to degrade cytokines and suppress inflammation (2017)
  • Author(s): Aly HH, Shiromoto F, Watashi K, Kato T, Chayama K, Wakita T
  • Organizer: The 65th Annual Meeting of the Japanese Society for Virology
• [Presentation] Membrane protein, embryonic lethal, abnormal vision, drosophila-like 1, interacts with NS5A and involves in hepatitis C virus replication (2017)
  • Author(s): Kao YT, Goto K, Aoyagi H, Hossam GE, Zheng X, Puig-Basagoiti F, Watashi K, Suzuki R, Yamagoe S, Dohmae N, Suzuki T, Okushin K, Tsutsumi T, Yotsuyanagi H, Moriya K, Koike K, Suzuki T, Wakita T, Aizaki H
  • Organizer: The 65th Annual Meeting of the Japanese Society for Virology
• [Presentation] The bidirectional interplay of hepatitis B virus and cell-death inducing DFF45-like effectors (2017)
  • Author(s): Kasai H, Yasumoto J, Yamashita A, Tanaka T, Watashi K, Wakita T, Otoguro T, Moriishi K
  • Organizer: The 65th Annual Meeting of the Japanese Society for Virology
• [Presentation] Novel crosstalk of Xenobiotic Response with Lipid Biosynthesis Pathway regulates the Host Permissiveness to the Hepatitis C Virus Production (2017)
  • Author(s): Ohashi H, Nakajima S, Kim S, Fukasawa M, Suzuki R, Aizaki H, Sugawara F, Ohtani N, Wakita T, Watashi K
  • Organizer: The 65th Annual Meeting of the Japanese Society for Virology
• [Presentation] Identification of a hepatitis B virus internalization inhibitor with a novel mode of action (2017)
  • Author(s): Fukano K, Tsukuda S, Suzuki R, Aizaki H, Wakita T, Ogasawara Y, Watashi K
  • Organizer: The 65th Annual Meeting of the Japanese Society for Virology
• [Presentation] Interaction of hepatitis B virus core protein with microtubles is important for the efficient capsid formation (2017)
  • Author(s): Iwamoto M, Cai D, Sugiyama M, Suzuki R, Aizaki H, Ryo A, Ohtani N, Tanaka Y, Mizokami M, Wakita T, Watashi K
  • Organizer: The 65th Annual Meeting of the Japanese Society for Virology
• [Presentation] Phosphorylation of protein kinase R and translational suppression by NS5B protein of hepatitis C virus (2017)
  • Author(s): Suzuki R, Matsuda M, Shimoike T, Watashi K, Aizaki H, Kato T, Suzuki T, Wakita T
  • Organizer: The 65th Annual Meeting of the Japanese Society for Virology
• [Presentation] Generation of hepatitis C virus vaccine antigen based on Japanese encephalitis virus subviral particles (2017)
  • Author(s): Yato K, Matsuda M, Watanabe N, Nakajima S, Fujimoto A, Watashi K, Aizaki H, Kato T, Tamura K, Wakita T, Suzuki R
  • Organizer: The 65th Annual Meeting of the Japanese Society for Virology
• [Presentation] Establishment of quantification method for the efficacy of anti-hepatitis virus combination treatments (2017)
  • Author(s): Watashi K, Koizumi Y, Ohashi H, Nakajima S, Tanaka Y, Wakita T, Perelson AS, Iwami S
  • Organizer: The 65th Annual Meeting of the Japanese Society for Virology
• [Presentation] NTCPを利用したHBV創薬研究およびNTCPを介したHBV侵入機構の解析 (2017)
  • Author(s): 九十田千子, 渡士幸一, 小嶋聡一, 脇田隆字
  • Organizer: 2017年度 生命科学系合同年次大会
  • Invited
• [Presentation] Involvement of cell-death inducing DFF45-like effectors in HBV replication (2017)
  • Author(s): Yasumoto J, Kasai H, Yoshimura K, Otoguro T, Watashi K, Wakita T, Yamashita A, Tanaka T, Takeda S, Moriishi K
  • Organizer: 5th JAPAN-TAIWAN-KOREA HBV Research Symposium 2017
  • Int'l Joint Research
• [Presentation] Hepatitis B virus capsid formation is regulated by microtubules and their posttranslational modification (2017)
  • Author(s): Iwamoto M, Sugiyama M, Suzuki R, Aizaki H, Tanaka Y, Mizokami M, Ohtani N, Wakita T, Watashi K
  • Organizer: 5th JAPAN-TAIWAN-KOREA HBV Research Symposium 2017
  • Int'l Joint Research
• [Presentation] B型肝炎ウイルスの内在化阻害剤の同定 (2017)
  • Author(s): Fukano K, Tsukuda S, Suzuki R, Aizaki H, Wakita T, Ogasawara Y, Watashi K
  • Organizer: 第14回ウイルス学キャンプin湯河原
• [Presentation] Chemical array system for identifying anti-hepatitis B virus entry inhibitors (2017)
  • Author(s): 九十田千子, 金子学, 渡士幸一, 小嶋聡一, 脇田隆字
  • Organizer: 第14回ウイルス学キャンプin湯河原
• [Presentation] Crosstalk of Xenobiotic Metabolism Pathway with Lipid Metabolism regulates the Host Permissiveness to Hepatitis C Virus Production (2017)
  • Author(s): Ohashi H, Nakajima S, Kim S, Suzuki R, Aizaki H, Fukasawa M, Kamisuki S, Sugawara F, Ohtani N, Wakita T, Watashi K
  • Organizer: 第14回ウイルス学キャンプin湯河原
• [Presentation] 培養細胞を用いたB型肝炎ウイルス cccDNAの動態解析 (2017)
  • Author(s): Iwamoto M, Kakizoe Y, Iwami S, Watashi K
  • Organizer: 第14回ウイルス学キャンプin湯河原
• [Presentation] Antiviral effect of bile acid derivatives on hepatitis B virus infection (2017)
  • Author(s): Okumura A, Ito K, Watashi K, Takeuchi J, Inoue R, Suzuki S, Sakamoto K, Okutsu S, Umezawa K, Wakita T, Yoneda M
  • Organizer: 2017 International HBV Meeting The Molecular Biology of Hepatitis B Viruses
  • Int'l Joint Research
• [Presentation] Modulation of HBV life cycle by sulforaphane (2017)
  • Author(s): Okamura H, Sasai M, Nio Y, Watashi K, Wakita T, Hijikata M
  • Organizer: 2017 International HBV Meeting The Molecular Biology of Hepatitis B Viruses
  • Int'l Joint Research
• [Presentation] Fatty acids with certain carbon chain length can support hepatitis B virus production (2017)
  • Author(s): Okamura H, Nio Y, Watashi K, Wakita T, Hijikata M
  • Organizer: 2017 International HBV Meeting The Molecular Biology of Hepatitis B Viruses
  • Int'l Joint Research
• [Presentation] An Epidemiology Study of Acute Hepatitis B in Japan, April 1999 ~ December 2015 (2017)
  • Author(s): Zheng X, Aoyagi H, Hossam G, Zaitsu T, Puig-Basagoiti F, Kao Y, Watashi K, Suzuki R, Takahashi T, Sunagawa T, Oishi K, Wakita T, Aizaki H
  • Organizer: 2017 International HBV Meeting The Molecular Biology of Hepatitis B Viruses
  • Int'l Joint Research
• [Presentation] Structural Proteins of Hepatitis C virus Genotype 3a S310 Strain Permit Claudin-1- independent Entry (2017)
  • Author(s): Suzuki R, Yato K, Tosaka T, Hmwe SS, Matsuda M, Watashi K, Aizaki H, Fukasawa M, Matsuura Y, Wakita T
  • Organizer: 24th International Symposium on Hepatitis C Virus and Related Viruses
  • Int'l Joint Research
• [Presentation] NS5A-Associated Membrane Protein, Embryonic Lethal, Abnormal Vision, Drosophila-Like 1, Involves in Hepatitis C Replication (2017)
  • Author(s): Kao YT, Goto K, Aoyagi H, Hossam GE, Zheng X, Puig-Basagoiti F, Watashi K, Suzuki R, Yamagoe S, Dohmae N, Suzuki T, Okushin K, Tsutsumi T, Yotsuyanagi H, Moriya K, Koike K, Suzuki T, Wakita T, Aizaki H
  • Organizer: 24th International Symposium on Hepatitis C Virus and Related Viruses
  • Int'l Joint Research
• [Presentation] Novel Crosstalk of Xenobiotic Response with Lipid Metabolism Regulates the Host Permissiveness to Hepatitis C Virus Production (2017)
  • Author(s): Ohashi H, Nakajima S, Kim S, Suzuki R, Aizaki H, Fukasawa M, Kamisuki S, Sugawara F, Ohtani N, Wakita T, Watashi K
  • Organizer: 24th International Symposium on Hepatitis C Virus and Related Viruses
  • Int'l Joint Research
• [Presentation] Broad antiviral activities of acidic polysaccharides isolated from marine algae (2017)
  • Author(s): Ueno M, Nogawa M, Siddiqui R, Watashi K, Wakita T, Kato T, Oda T, Ariumi Y
  • Organizer: The 65th Annual Meeting of the Japanese Society for Virology
• [Presentation] Analysis of HBV life cycle in the HepG2 expressing human NTCP cell line (2017)
  • Author(s): Rahayu R, Ohsaki E, Honda T, Okamoto T, Watashi K, Ueda K
  • Organizer: The 65th Annual Meeting of the Japanese Society for Virology
• [Presentation] 50.Fatty acids synthesized from cellular fatty acid synthesis pathway play roles in hepatitis B virus production (2017)
  • Author(s): Okamura H, Nio Y, Watashi K, Wakita T, Hijikata M
  • Organizer: The 65th Annual Meeting of the Japanese Society for Virology
• [Presentation] Human hepatic stellate cells are permissive for hepatitis C virus infection/replication and play important roles in fibrosis (2017)
  • Author(s): Zheng X, Zaitsu T, Aoyagi H, Matsuda M, Watanabe N, Fujimoto A, Watashi K, Suzuki R, Fukuhara T, Matsuura Y, Ichinose S, Wake K, Suzuki T, Iijima H, Yokoyama H, Masaki T, Matsuura T, Tamura K, Wakita T, Aizaki H
  • Organizer: The 65th Annual Meeting of the Japanese Society for Virology
• [Presentation] Abnormal hepatocellular organelles remain to be observed in sustained virological response patients (2017)
  • Author(s): Aoyagi H, Iijima H, Puig-Basagoiti F, Zheng X, Kao YT, Hossam GE, Matsuda M, Watashi K, Suzuki R, Masaki T, Aizawa N, Shimada N, Kato K, Tsubota A, Mimata A, Sakamaki Y, Ichinose S, Wake K, Wakita T, Aizaki H
  • Organizer: The 65th Annual Meeting of the Japanese Society for Virology
• [Presentation] Screening of FDA-approved drugs activating cyclic GMP-AMP synthase promoter (2017)
  • Author(s): Imai H, Dansako H, Ueda Y, Satoh S, Watashi K, Wakita T, Kato N
  • Organizer: The 65th Annual Meeting of the Japanese Society for Virology
• [Presentation] Establishment of a neutralization assay for multiple flaviviruses based on single-round infectious particles (2017)
  • Author(s): Matsuda M, Yamanaka A, Yoshii K, Watashi K, Aizaki H, Konishi E, Takasaki T, Wakita T, Suzuki R
  • Organizer: The 65th Annual Meeting of the Japanese Society for Virology
• [Presentation] Identification of a new class of HBV and HDV entry inhibitor, proanthocyanidin (2017)
  • Author(s): Tsukuda S, Watashi K, Kojima S, Wakita T
  • Organizer: AASLD The Liver Meeting 2017
  • Int'l Joint Research
• [Book] Encyclopedia of Signaling Molecules, 2nd Edition (2017)
  • Author(s): Tsukuda S, Iwamoto M, Watashi K
  • Total Pages: 3591-3599
  • Publisher: Springer Nature