Role of CCL28 for mucosal immunity

2016 Fiscal Year Final Research Report

• Project/Area Number: 26460582
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Immunology
• Research Institution: Kindai University
• Principal Investigator: YOSHIE Osamu 近畿大学, 医学部, 教授 (10166910)
• Co-Investigator(Renkei-kenkyūsha): NAKAYAMA Takashi 近畿大学, 薬学部, 教授 (60319663)
• Research Collaborator: TSUNODA Ikuo 近畿大学, 医学部, 教授 (00261529)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: ケモカイン / CCL28 / 腸管免疫 / IgA / 腸内細菌叢

Outline of Final Research Achievements

CCL28 is expressed in the mucosal tissues and to attract IgA-antibody secreting cells (IgA-ASCs) via CCR10. CCL28 has an antimicrobial activity against microbes in vitro. However, in vivo evidence for the role of CCL28 in the mucosal immunity remains scanty. We generated CCL28-deficient mice and demonstrated that CCL28-deficient mice showed reduced numbers and altered distribution of IgA-ASCs in the colon. The IgA contents in the fecal extracts were low. The average amounts of IgA secreted by a single IgA-ASC isolated from the lamina propria of the colon was reduced. Furthermore, the 16S rRNA sequencing analysis of feces revealed an increase of the Class Bacilli. Consistent with the low IgA production and altered microbiota in the colon, CCL28-deficient mice had aggravated colitis upon treatment with dextran sulfate, which was ameliorated by oral antibiotics. Therefore, CCL28 has an role in the mucosal immunity of the colon as a chemoattractant with a direct antimicrobial activity.

Free Research Field

免疫学