Developing human lipid profiling methods using mass spectrometry and their applications in clinical laboratory tests

2016 Fiscal Year Final Research Report

• Project/Area Number: 26460647
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Shinshu University
• Principal Investigator: HIDAKA Hiroya 信州大学, 学術研究院保健学系, 准教授 (10362138)
• Research Collaborator: YAMAMOTRO Hiroshi ; NAKAMURA Tomohiko ; HIDAKA Eiko ; SUGANO Mitsutoshi ; KAWASAKI Kenji ; ISHIMINE Nau ; KIDOKUCHI Shuhei ; NISHIJIMA Fumie ; HORI Atsushi
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 質量分析 / ヒト血液 / 脂質分子種

Outline of Final Research Achievements

Phospholipids and their metabolites function as regulators of lipid metabolism and are associated with various diseases. Lipid molecular species in human biomedical materials, such as blood and bronchoalveolar lavage fluid, were analyzed using the methods of gel chromatography and Matrix assisted laser desorption/ionization time of flight mass spectrometry(MALDI-TOF MS), Liquid chromatography MS, and Gas chromatography MS.Furthermore, we generated lipid species profiles of healthy subjects and then visualized these molecular species. We also showed that these methods for lipid molecular species analysis can be used in clinical laboratory tests. We are currently accumulating clinical data to identify specific lipid molecular species associated with pathological conditions.

Free Research Field

脂質分析学