Development of the diagnostic method for detection of multidrug-resistant Pseudomonas aeruginosa and Acinetobacter baumannii using efflux pumps as a target, and its application to infection control

2016 Fiscal Year Final Research Report

• Project/Area Number: 26460657
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Tokai University
• Principal Investigator: ASAI Satomi 東海大学, 医学部, 准教授 (60365989)
• Co-Investigator(Renkei-kenkyūsha): MIYACHI Hayato 東海大学, 医学部, 教授 (20174196)
• Research Collaborator: YOSHIHARA Eisaku 東海大学, 工学部, 客員准教授 ; OHSHIMA Toshio 東海大学, 医学部付属病院, 臨床検査技師
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 緑膿菌 / アシネトバクター / 多剤耐性菌 / 薬剤排出ポンプ / Bam / 簡易迅速検査 / 感染制御 / real time RT-PCR

Outline of Final Research Achievements

The mutations in the genes responsible for the development of antimicrobial resistance were investigated in the drug-resistant Pseudomonas aeruginosa and Acinetobacter baumannii which showed resistance to at least 2 among imipenem, amikacin and ciprofloxacin. As a result, the contribution of the efflux pumps was found involved in almost all drug-resistant strains. On the other hand, the complex protein which supplied s peptides for efflux pumps did not significantly increased.
The monitoring of the efflux pumps of P. aeruginosa and Acinetobacter baumannii was suggested to be useful in infection control.

Free Research Field

臨床検査医学