Analyzing role of non-coding RNA in multiple myeloma by using next generation sequnecer

2016 Fiscal Year Final Research Report

• Project/Area Number: 26460665
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Gunma University
• Principal Investigator: Handa Hiroshi 群馬大学, 医学部附属病院, 講師 (90282409)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 多発性骨髄腫 / non coding RNA / 次世代シークエンサー / 髄外形質細胞腫 / ストレス

Outline of Final Research Achievements

We analyzed multiple myeloma (MM), precancerous stage MGUS, refractory extramedullary plasmacytoma (EMM) by the next-generation sequencer how non coding RNA was associated with disease progression. As a result, MALAT1 and NEAT1 expressed higher in MM and EMM than in MGUS, particularly, MALAT1 expressed markedly higher in EMM with several thousand fold. We knocked down MALAT1 in MM cell line, but the change was not found in a cell proliferation, cell motility gene expression. MALAT1 and NEAT1 expression increased by bortezomib and doxorubicin. It was suggested that induced MALAT1 and NEAT1 by drug stress were associated with EMM formation.

Free Research Field

血液内科学　血液検査学