Genetic analysis of peritoneal dissemination of gastrointestinal cancer

2016 Fiscal Year Final Research Report

• Project/Area Number: 26460936
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Nagoya University
• Principal Investigator: Maeda Osamu 名古屋大学, 医学系研究科, 特任講師 (20378053)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 胃癌 / 化学療法 / 腹膜播種

Outline of Final Research Achievements

The purpose of the present study is to analyze gene alteration accompanied with acquisition of drug-resistance against chemotherapy.
During chemotherapy, cancer cells that are sensitive and resistance to chemotherapy were obtained by repeated paracentesis from gastric cancer patients with peritoneal dissemination. Cancer cells were selectively cultured for short duration eliminating normal cells including blood cells and mesothelial cells. Microarray analysis of gene expression and DNA methylation were performed using the cultured cancer cells. Furthermore, we established drug-resistant cells of gastric cancer cell line AGS, and performed genome-wide analysis. We extracted gene alteration with possible relationship with drug resistance.

Free Research Field

がん薬物療法