Functional analysis of a novel bile acid-sensor in the mouse upper gastrointestinal tract.

2016 Fiscal Year Final Research Report

• Project/Area Number: 26460946
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Nagoya City University
• Principal Investigator: SHIKANO Michiko 名古屋市立大学, 大学院医学研究科, 研究員 (70405190)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: ASIC5 / マウス食道 / RT-PCR / ウェスタンブロット / 免疫組織化学 / ヒト正常食道上皮細胞 / TGR5 / 特異抗体

Outline of Final Research Achievements

Acid-sensing ion channel 5 (ASIC5；also called BLINaC or BASIC) is a novel bile acid-sensitive ion channel, which is expressed in the epithelial cells that line bile ducts (Wiemuth et al, 2012), but the expression in the upper digestive tract is unknown. We thus examined the expression of ASIC5 in the mouse esophagus. RT-PCR analysis found that the short fragments of ASIC5 transcripts were abundantly detected in the middle and lower regions. Full length PCR cloning analysis identified a novel splicing variant from the mouse esophagus along with the registered ASIC5 transcripts. We also examined the expression of acid-sensitive G protein-coupled receptor TGR5 known as another candidate in a human normal esophageal epithelial cell line HET-1A, but apparent expression was not detected. ASIC5 may participate in physiological and pathophysiological functions of the esophagus, although the expression of ASIC5 protein was not confirmed because of lack of antibodies specific to ASIC5.

Free Research Field

医歯薬学