2016 Fiscal Year Final Research Report
Study for cardioprotection of omega-3 polyunsaturated fatty acid
Project/Area Number |
26461065
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Cardiovascular medicine
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Research Institution | Hamamatsu University School of Medicine |
Principal Investigator |
Sato Hiroshi 浜松医科大学, 医学部附属病院, 講師 (30293632)
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Keywords | 不飽和脂肪酸 / 心筋細胞 / 冠動脈疾患 / ミトコンドリア |
Outline of Final Research Achievements |
(Basic study) When differentiated H9c2 myocytes were exposed to palmitate (PAL), the myocytes showed activation of caspases 3/7, depolarization of mitochondrial membrane potential, depletion of intracellular ATP, and enhancement of reactive oxygen species production. PAL enhanced mitochondrial fragmentation with increased dynamin-related protein-1 (Drp1) expression. Eicosapentaenoic acid (EPA) ameliorated the PAL-induced lipotoxicity via AMP-activated protein kinase activation, which suppressed mitochondrial fragmentation and Drp1 expression. (Clinical study) Patients with fish intake >=3 times/week showed higher EPA, DHA levels and EPA/AA ratio than those with <3 times/week. Both patients recommended fish intake and those treated with EPA showed decreases in serum MDA-LDLlevels after 2 years, but the extent did not differ. The increase in fish intake was as effective as the treatment with EPA for increases in serum omega-3 polyunsaturated fatty acids and a decrease in oxidative LDL.
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Free Research Field |
循環器内科
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