2016 Fiscal Year Final Research Report
The cross-talk between innate immunity and acquired immunity in exacerbation of airway inflammation in asthmatic attack
Project/Area Number |
26461186
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Respiratory organ internal medicine
|
Research Institution | Hamamatsu University School of Medicine |
Principal Investigator |
|
Research Collaborator |
MORI KAZUTAKA 浜松医科大学, 医学部, 大学院生
YAMANAKA KATSUMASA 浜松医科大学, 医学部, 大学院生
|
Project Period (FY) |
2014-04-01 – 2017-03-31
|
Keywords | 気管支喘息 / 気道炎症 / IL-17A / Toll like receptor / 炎症性サイトカイン / 自然免疫 / 獲得免疫 |
Outline of Final Research Achievements |
The interaction between IL-17A and TLR3 activation in airway epithelium remains poorly understood. In this study, we demonstrated that IL-17A and polyI:C, the ligand of TLR3, synergistically induced proinflammatory cytokine and chemokine (G-CSF, IL-8) mRNA and protein expression. Inhibition of the NF-κB pathway using NF-κB p65 siRNA, or BAY11-7082 (IκB-α inhibitor) attenuated the IL-17A/polyI:C-induced synergistic expression of proinflammatory cytokines. Knockdown of IRF3 using siRNA also decreased the synergistic gene expression. In western blotting analysis, co-treatment with IL-17A and polyI:C augmented IκB-α phosphorylation compared to polyI:C treatment alone. These findings demonstrate that IL-17A and TLR3 activation act in concert to potentiate proinflammatory responses in the airway epithelium via NF-κB/IRF3 activation, and that enhanced activation of the NF-κB pathway play a key role in the synergism.
|
Free Research Field |
呼吸器内科学
|