2016 Fiscal Year Final Research Report
a trial for generation of parietal podocytes
| Project/Area Number |
26461241
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Kidney internal medicine
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| Research Institution | Jikei University School of Medicine |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
Matsusaka Taiji 東海大学, 医学部・総合医学研究所, 教授 (50317749)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | BMP |
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| Outline of Final Research Achievements |
After the parietal epithelial cell-specific expression vector (pPEC-rtTA) was constructed and injected into mouse fertilized eggs, we obtained eight founder mice carrying pPEC-rtTA. However, we could not establish activator mouse lines from these mice. Therefore, we examined whether the parietal podocytes were formed in the adult Podocine-rtTA/TetO-Noggin administered Dox for a long time. The results indicated that parietal podocytes were not observed with respect to morphology and expression of several podocyte-markers. In contrast, proximal tubular damages and interstitial edema were intensely seen in the mice.
Hence, in contrast to the prenatal period, inhibition of BMP signal in glomeruli in adulthood cannot induce parietal podocytes.
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| Free Research Field |
腎臓内科学
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