Elucidation of pathogenesis of sporadic ALS using motor neuron specific-dynactin-1 knockout mice

2016 Fiscal Year Final Research Report

• Project/Area Number: 26461269
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neurology
• Research Institution: Nagoya University
• Principal Investigator: KAWAI Kaori 名古屋大学, 医学系研究科, 研究員 (80398007)
• Co-Investigator(Renkei-kenkyūsha): KATSUNO Masahisa 名古屋大学, 大学院医学系研究科, 教授 (50402566)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: ALS / dynactin-1 / ノックアウトマウス / 神経変性疾患

Outline of Final Research Achievements

To elucidate the pathogenesis of sporadic amyotrophic lateral sclerosis (ALS), we generated motor neuron-specific dynactin-1 knockout mice. In this study, we could not identify ubiquitinated protein included in the ubiquitin-positive aggregates in motor neurons of the spinal anterior horn because of less amount of targeted proteins. There was a need to improve the method for recovering ubiquitinated protein. In the dynactin-1 knockout mice, glial activation detected before appearance of neurodegenerative symptoms, such as tremor and reduction in motor function.

Free Research Field

神経内科学