GDP-bound Rab27a regulates endocytosis by mediating vATPase-dependent regulation of pH

2016 Fiscal Year Final Research Report

• Project/Area Number: 26461342
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Metabolomics
• Research Institution: Oita University
• Principal Investigator: Kimura Toshihide 大分大学, 医学部, 准教授 (60404373)
• Co-Investigator(Renkei-kenkyūsha): ISHIZAKI Toshimasa 大分大学, 医学部, 教授 (70293876) ; TERABAYASHI Takeshi 大分大学, 医学部, 助教 (40452429)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 糖尿病 / インスリン分泌 / Rab27a / エンドサイトーシス / 膵B細胞 / Gタンパク質 / メンブレントラフィック / エキソサイトーシス

Outline of Final Research Achievements

Impaired insulin secretion in response to glucose plays an important role in the pathogenesis of diabetes mellitus. Although endocytosis of secretory membranes after insulin release is essential for the tight control of insulin secretion, knowledge about this process in pancreatic beta-cells is still limited. Here, I searched for GDP-bound Rab27a interacting proteins and identified proton pumps. I found that GDP-bound Rab27a promotes the formation of the proton pump complex, which is required for its activity. These results suggest that GDP-bound Rab27a regulates endocytosis via acidification in endocytosed vesicles.

Free Research Field

分子糖尿病学