2016 Fiscal Year Final Research Report
Reconstitution of mononuclear phagocyte system following allogeneic hematopoietic stem cell transplantation
| Project/Area Number |
26461438
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 同種造血幹細胞移植 / マクロファージ / 移植片対宿主病 / GVHD / 貪食細胞 |
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| Outline of Final Research Achievements |
Reconstitution of tissue resident macrophages and inflammatory macrophages after allogeneic hematopoietic stem cell transplantation (SCT) were assessed using murine model of allogeneic bone marrow transplantation (BMT). We found that severe GVHD promoted replacement of tissue resident macrophages in the target organs by donor-derived macrophages on day +14 after allogeneic BMT. Contrary, it took at least a few months for reconstitution of donor macrophages after syngeneic BMT, suggesting that allogeneic response accelerated donor reconstitution of tissue resident macrophages. Next, we addressed turnover of inflammatory macrophages after SCT. Massive infiltration of inflammatory macrophages was found in the lung and skin, and depletion of these cells using monoclonal antibodies ameliorated graft versus host disease (GVHD) after allogeneic SCT. Our findings have important clinical implications with respect to establishment of tolerance and pathophysiology of GVHD after allogeneic SCT.
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| Free Research Field |
造血幹細胞移植学
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