2016 Fiscal Year Final Research Report
AU-rich element dependent mRNA regulation in functional change of rheumatoid arthritis synoviocyte
| Project/Area Number |
26461463
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Collagenous pathology/Allergology
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| Research Institution | Kurume University (2016)
Hiroshima University (2014-2015) |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | mRNA制御 / 3'UTR / AU-rich element / サイトカイン / 関節リウマチ / 滑膜細胞 / 発現制御 |
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| Outline of Final Research Achievements |
Anti-cytokine antibody therapy has proven the importance of cytokines as therapeutic targets. In this study, cytokines that regulate the metabolism of mRNA via AU rich element present in the 3 'untranslated region of mRNA were screened by using a reporter plasmid.
IL-1β enhanced the luciferase activity of the reporter having the 3 'UTR of CXCL2 mRNA. IL-1β leads to enhanced mRNA and protein expression of CXCL2 in synovial cells, which proved that CXCL2 mRNA is stabilized by IL-1β. Co-transfection of TTP with the reporter plasmid suppressed the luciferase activity with the 3′UTR of CXCL2 mRNA. It became clear that cytokine contributes to induction of an inflammatory gene by stabilizing the mRNA in addition to transcriptional up-regulation of the gene.
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| Free Research Field |
膠原病・アレルギー内科学
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