Isolation and epitope analysis of broadly neutralizing antibodies against Influenza viruses

2017 Fiscal Year Final Research Report

• Project/Area Number: 26461517
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Infectious disease medicine
• Research Institution: Fujita Health University
• Principal Investigator: Ohshima Nobuko 藤田保健衛生大学, 産学連携推進センター, 講師 (60387694)
• Co-Investigator(Renkei-kenkyūsha): HIRANO Daisuke 藤田保健衛生大学, 医学部, 助教 (70767110)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: 交差反応性 / ファージ抗体ライブラリー

Outline of Final Research Achievements

In this study, monoclonal antibodies that cross-react with antigenic variants of type A H3N2 influenza virus were isolated from a human phage antibody library and analyzed. The antibodies that bind to both type A and B influenza vaccines are included in these isolated antibodies, but there was no confirmation that the antibodies recognize hemagglutinin which is a target of virus neutralizing antibody. We also succeeded in isolating another antibodies cross-reactive with all antigenic variants of type A H3N2 influenza virus used in this study. If the epitope conserved in type A H3N2 virus strains is determined through the analysis of these antibodies, we believe that in the future it will lead to the development of a vaccine capable of inducing antibodies recognizing the epitope.

Free Research Field

分子生物学