2016 Fiscal Year Final Research Report
The structural analysis of the onset mechanism of cryopyrin-associated periodic syndrome
| Project/Area Number |
26461576
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Gifu University |
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Principal Investigator |
|
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| Research Collaborator |
TOCHIO HIDEHITO 京都大学
KIMURA TAKESHI 岐阜大学
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| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Keywords | 自己炎症性疾患 / 自然免疫 |
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| Outline of Final Research Achievements |
The purpose of this study was the recombinant protein expression, purification and molecular analyses of human NLRP3, in order to analysis the onset mechanism of cryopyrin-associated periodic syndrome. We tried to use the insect cell protein expression systems (silkworm or SF9 cells) with the various protein expression constructs. The protein expression of NLRP3 variants was confirmed, but they were insoluble. Therefore, we could not obtain the suitable protein samples for analyzing the function and structure.
In conclusion, our study revealed the quite difficulty of the large-scale protein purification of human NLRP3.
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| Free Research Field |
小児科学
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