Study of recurrence mechanism in super high-risk neuroblastoma

2016 Fiscal Year Final Research Report

• Project/Area Number: 26461591
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pediatrics
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: Tomoko Iehara 京都府立医科大学, 医学(系)研究科(研究院), 准教授 (20285266)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 神経芽腫 / スーパーハイリスク

Outline of Final Research Achievements

Genome analysis was performed from the initial tumor and metastatic recurrent tumor tissue of the super high-risk group neuroblastoma. Deletion of CDKN2A, which does not exist at the onset, was observed in metastatic recurrent tissues. We analyzed CDKN2A and Rsf-1 in 19 neuroblastoma cell lines by real-time PCR method, there is no correlation was found with expression of CDKN2A and expression of Rsf-1. However, expression of Rsf-1 tended to be higher in the MYCN-amplified cell line. Next, We analyzed Segmental chromosome Aberration (SCA), when Rsf-1 knockdown was performed using the neuroblastoma cell line SH-SY5Y with high expression of Rsf-1. We cannot observed any changes in SCA (Segmental chromosome Aberration) in this cell line.

Free Research Field

小児腫瘍学