Development of a novel therapy for perinatal brain injury with dedifferentiated fat cells

2016 Fiscal Year Final Research Report

• Project/Area Number: 26461630
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Embryonic/Neonatal medicine
• Research Institution: Nagoya University
• Principal Investigator: Sato Yoshiaki 名古屋大学, 医学部附属病院, 講師 (30435862)
• Co-Investigator(Renkei-kenkyūsha): MATSUMOTO TARO 日本大学, 医学部, 教授 (50366580) ; TSUHI MASAHIRO 独立行政法人国立循環器病研究センター, 再生医療部, 室長 (80579467)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 低酸素性虚血性脳症 / 幹細胞

Outline of Final Research Achievements

In the present study, we aimed to determine whether the outcome of HIE can be improved by DFAT cell treatment. 24 h after HI insult, DFAT cells were injected at 10^5 cells/pup into the right external jugular vein. The number of cells that stained positive for active caspase-3, ED-1, and 4-HNE decreased in the DFAT-treated group. The HI insult led to a motor deficit according to the rotarod treadmill and cylinder test, where it significantly affected the vehicle group, whereas no difference was confirmed between the DFAT and sham groups. According to in vitro experiments, the cell death rates in the DFAT-CM-treated cells were significantly lower than those in the controls. In the DFAT-CM, several neurotrophic factors, IGF-1、NGF、NT3 were detected, and these factors were increased in the ipsilateral brain after injection of DFAT. Our results indicate that intravenous injection with DFAT cells is effective for ameliorating HI brain injury, possibly via paracrine effects.

Free Research Field

新生児学