The verification of the fetal origins of adult disease theory using human iPS cell pancreatic differentiation system

2016 Fiscal Year Final Research Report

• Project/Area Number: 26461638
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Embryonic/Neonatal medicine
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: shiraki nobuaki 東京工業大学, 生命理工学院, 准教授 (70448520)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: DOHaD / Barker説 / 生活習慣病胎児期発症説 / iPS / アミノ酸

Outline of Final Research Achievements

Low birth weight is strongly predictive of lifestyle related disease. Animal study indicated that fetal malnutrition induce fetuses with growth retardation have a decreased beta cell mass, which persists into adulthood and causes glucose intolerance. However, The verification of this malnutrition’s effect on human fetuses was difficult. The aim of this study is checking the effect of fetal malnutrition on human using human iPS cell pancreatic differentiation methods. In this study, I have found that important amino acids which regulate pancreatic differentiation at narrow time windows using novel pancreatic differentiation methods established in this study. As a results of amino acid deprivation during pancreatic endocrine differentiation, the number of pancreatic beta cell is significantly decreased at final stage. These results indicated that iPS cell differentiation systems is useful tools for analysis of fetal origins of adult disease theory.

Free Research Field

幹細胞制御学