Hypotonic stimulation enhances cellular uptake and cytocidal effect of anticancer drug in gastric cancer cells

2016 Fiscal Year Final Research Report

• Project/Area Number: 26461988
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: Shiozaki Atsushi 京都府立医科大学, 医学(系)研究科(研究院), 助教 (40568086)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 胃癌

Outline of Final Research Achievements

Intraperitoneal injection of paclitaxel (PTX) is the promising treatment option for peritoneal dissemination of gastric cancer (GC). This study examined whether hypotonic stimulation enhances cellular uptake and cytocidal effect of PTX in GC cells. After MKN45 cells were incubated with Oregon green 488 PTX in the iso- or hypotonic solution, cellular uptake of PTX was determined by flow cytometer. After cells were incubated with PTX for 1h under iso- or hypotonic condition, the number of viable cells was counted at 48h after stimulation in order to examine cytocidal effect of PTX. Since the fluorescence intensity of a MKN45 cell was significantly higher in the hypotonic condition, hypotonic stimulation enhanced cellular uptake of PTX. The number of viable MKN45 cells after stimulation was smaller in the hypotonic condition, suggesting that hypotonic stimulation enhanced cytocidal effect of PTX. Hypotonic stimulation enhances cellular uptake and cytocidal effect of PTX in MKN45 cells.

Free Research Field

消化器外科学