2016 Fiscal Year Final Research Report
Analysis of CD11c+ cells in human intestinal lamina propria
| Project/Area Number |
26462014
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Digestive surgery
|
|---|
| Research Institution | Osaka University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Keywords | ヒト腸管粘膜固有細胞 / CD103+CD14-細胞 / 炎症性腸疾患 / 潰瘍性大腸炎 |
|---|
| Outline of Final Research Achievements |
Normal intestinal mucosa was obtained from intact sites of colorectal cancer patients. Non-inflamed and inflamed colonic tissues were obtained from surgically resected specimens of UC patients. Among Lin-CD45+HLA-DRhigh intestinal lamina propria cells, CD103+ cells were sorted and analyzed. CD103+ cells in the normal colon showed lower expression of pro-inflammatory cytokines than CD103-CD14+ cells. Co-culture with naïve T cells revealed that CD103+ DCs generated Treg cells. CD103+ DCs from UC patients did not generate Treg cells, but they induced Th1/Th2/Th17 cells and showed higher expression of IL6, IL23A, IL12p35, and TNF. These findings show how human CD103+ DCs could contribute to the pathogenesis of UC.
|
| Free Research Field |
医歯薬学
|
