Clinical implication of Farnesoid-X-receptor agonist in liver regeneration after hepatectomy

2016 Fiscal Year Final Research Report

• Project/Area Number: 26462047
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Kumamoto University
• Principal Investigator: TAKAMORI Hiroshi 熊本大学, 医学部附属病院, 非常勤診療医師 (90363514)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: FXR agonist / 肝再生 / 肝切除

Outline of Final Research Achievements

Mouse model of 70 % hepatectomy was established. Protocol was prepared for the experiment with FXR agonist. In clinical experiment with paraffin embedded section, immunohistochemistry was performed with FXR antibody (PPMX corp., Clone No. A9033A). Staining pattern was appropriate and nuclear localization of FXR was confirmed. On the other hand, nuclear localization of FXR in more than 50% of hepatocytes in background liver was seen in almost all samples (n > 50). Therefore, it was hard to assign them into two groups with/without FXR activation. Other methodology is required for evaluating activation status of FXR in hepatocytes.

Free Research Field

消化器外科学（特に肝領域）