2016 Fiscal Year Final Research Report
Assessment of proliferation and apoptotic rate of tumor budding cells in pancreatic ductal adenocarcinoma.
| Project/Area Number |
26462056
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Niigata University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 簇出 / 増殖能 / アポトーシス / 膵癌 |
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| Outline of Final Research Achievements |
Tumor budding is a prognostic factor in PDAC. Budding cancer cells in AE1/AE3 immunostaining were counted significantly more than in HE staining (P < 0.001). High grade budding evaluated with both standard HE and AE1/AE3 staining correlated significantly with poor overall survival and the histological grade. Multivariate analysis revealed that the margin status, lymph node metastasis, the high grade budding with proliferative potency assessed by AE1/AE3 and Ki-67 double immunostaining were independent prognostic factors. The cumulative 2-year survival rates were 63.6% for patients with negative immunostaining of tumor budding for Ki-67, 46.9% for patients with positive of low grade budding and 0% for patients with positive of high grade budding (P < 0.001).The average percentage of cases showing Ki-67 positivity was 73% and the average percentage of cases showing TUNEL positivity was only 7%, suggesting decreased apoptosis at the budding cancer cells at invasive front of the tumor.
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| Free Research Field |
消化器外科
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