2016 Fiscal Year Final Research Report
Regulation of extracellular matrix remodeling induced by stellate cells and immune cells in the pancreatic tumor microenvironment
| Project/Area Number |
26462062
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 細胞外マトリックス / リモデリング / 膵星細胞 / 免疫細胞 / 低酸素環境 / PLOD2 / 脂肪由来幹細胞 |
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| Outline of Final Research Achievements |
The aim of this study was to clarify the mechanism and contribution of stellate cells and immune cells in of remodeling extracellular matrix of pancreatic cancer. We isolated activated stellate cells (PSCs) from human pancreatic cancer and analyzed the structure of collagen matrix produced by PSCs. Under hypoxic condition, the alignment of collagen fiber became parallel and enhanced motility of pancreatic cancer. We identified PLOD2 as a major collagen modifying enzyme under hypoxia. We also identified collagen matrix produced by PSCs was denser and stiffer than that of adipose tissue derived stromal cells, which means heterogeneity of stromal cells in pancreatic cancer influences mechanical structure of extracellular matrix in pancreatic cancer.
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| Free Research Field |
医歯薬学
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