2016 Fiscal Year Final Research Report
Synchrotron radiation pulmonary micro-angiography for evaluating micro-vasculature remodeling and endothelial dysfunction in pulmonary arterial hypertension.
| Project/Area Number |
26462081
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Cardiovascular surgery
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 肺高血圧症 / 放射光微小血管撮影法 / シアストレス |
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| Outline of Final Research Achievements |
We have previously established a synchrotron radiation pulmonary micro-angiography (SRPA) in an in-vivo rat model. In this study, we aimed to qualify micro-vascular remodeling in crotaline induced pulmonary arterial hypertension (PAH) rats. To determine the association of endothelial function to the vascular remodeling, local expression of endotheline-1(ET-1), endothelial nitric oxide synthase(eNOS) was evaluated by immunohistochemical staining. Microvascular density measured by SRPA was significantly decreased in the PAH rats. eNOS expression was decreased and ET-1 expression was increased significantly in the PAH rats. SRPA is a useful method to visualize decreased and narrowed pulmonary micro-vasculature in the PAH rats. Increased ET-1 expression may contribute to a proliferation and remodeling of pulmonary arterioles. Also, decreased eNOS expression may associate with vasospasm of pulmonary arterioles induced by endothelial dysfunction due to increased shear stress in PAH.
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| Free Research Field |
肺高血圧症
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