Effect of pericyte on the blood-brian barrier damage due to the hyperglycemia

2017 Fiscal Year Final Research Report

• Project/Area Number: 26462166
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neurosurgery
• Research Institution: Nagasaki University
• Principal Investigator: HAYASHI Kentaro 長崎大学, 医歯薬学総合研究科(医学系), 客員研究員 (40404222)
• Co-Investigator(Kenkyū-buntansha): 出雲 剛 長崎大学, 病院(医学系), 講師 (40343347) ; 諸藤 陽一 長崎大学, 病院(医学系), 助教 (40437869) ; 福田 修志 長崎大学, 病院(医学系), 医員 (40646577)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: blood-brain barrier

Outline of Final Research Achievements

GLP-1 increased transendothelial electrical resistance and decreased the permeability of sodium fluorescein in RBECs in a dose- and time-dependent manner. The effects on these barrier functions were significantly reduced in the presence of the GLP-1 receptor antagonist exendin-3 (9-39) and the protein kinase A (PKA) inhibitor H-89. Western blot analysis showed that GLP-1 increased the amount of occludin and claudin-5. GLP-1 analogs are approved for treatment of type 2 diabetes mellitus, and thus, we examined the effects of GLP-1 on hyperglycemia-induced BBB damage. GLP-1 inhibited the increase in production of reactive oxygen species under hyperglycemia conditions and improved the BBB integrity induced by hyperglycemia. As GLP-1 stabilized the integrity of the BBB, probably via cAMP/PKA signaling, the possibility that GLP-1 acts as a BBB-protective drug should be considered.

Free Research Field

脳神経外科学