Therapeutic Effects of Intravenous Infusion of MSCs in Hypoxic-Ischemic Encephalopathy Rat

2016 Fiscal Year Final Research Report

• Project/Area Number: 26462168
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neurosurgery
• Research Institution: Sapporo Medical University
• Principal Investigator: Sakai Takuro 札幌医科大学, 医学部, 研究員 (80639229)
• Co-Investigator(Renkei-kenkyūsha): HONMOU Osamu 札幌医科大学, 医学部, 教授 (90285007)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 低酸素性虚血性脳症 / 骨髄幹細胞 / 動物モデル

Outline of Final Research Achievements

We aimed to investigate the effect of mesenchymal stem cells (MSCs) transplantation in a rat model of neonatal hypoxic-ischemic encephalopathy (HIE) by histological, MRI, and behavioral analysis. At postnatal day 7, rats were underwent HI by left common carotid artery occlusion followed by 120 min hypoxia (8% oxygen). At postnatal day 10, transplantation of MSCs　was performed by intravenous injection. Compared with the vehicle (control) group, the MSC infused group showed a significant improvement in athletic performance by the evaluation with the beam walk test. The MRI also demonstrated that the MSC infused group showed a significant reduction of the ischemic volume compared to the vehicle group. Collectively, intravenous infusion of MSCs might provide therapeutic efficacy in a rat model of HIE.

Free Research Field

新生児学