Fluorescent tumor cell detection by tumor-specific virus VSV for precise osteosarcoma resection

2016 Fiscal Year Final Research Report

• Project/Area Number: 26462267
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Hiroshima University
• Principal Investigator: KUBO Tadahiko 広島大学, 医歯薬保健学研究院(医), 准教授 (70397959)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 骨肉腫 / 蛍光イメージング / 腫瘍溶解性ウイルス / 近赤外線蛍光蛋白

Outline of Final Research Achievements

Recombinant VSV expressing katushka was generated using the established method of reverse genetics. Five to six weeks old C3H mice were implanted subcutaneously with 1x107 osteosarcoma cells LM8 on the right hind leg. We observed tumor-specific fluorescence in vitro using fluorescence microscopy（BZ-9000、keyence）and in osteosarcoma-bearing mice using Bio-fluorescence imaging system （WinLight32, Berthold）. Using this imaging system, tumor wide resection was performed safely and precisely and circulating tumor cells were eliminated securely. In conclusion, this study proved the possibility of the new fluorescence imaging system to apply to the advanced therapy of primary lesion and circulating tumor cells.

Free Research Field

骨軟部腫瘍学