Establishment of novel osteochondral allografting combined with growth factor- collagen-binding domain fusion technology

2016 Fiscal Year Final Research Report

• Project/Area Number: 26462277
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Kitasato University
• Principal Investigator: Onuma Kenji 北里大学, 医学部, 講師 (80348557)
• Co-Investigator(Renkei-kenkyūsha): MATSUSHITA Osamu 岡山大学, 医歯(薬)学総合研究科, 教授 (00209537)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 同種骨軟骨移植 / 成長因子固相化技術

Outline of Final Research Achievements

We investigated study for improved osteochondral allografting combined with basic fibroblast growth factor (bFGF)- collagen-binding domain (CBD) fusion protein. In the preliminary study, optimal reaction time for binding bFGF-CBD to rat osteochondral tissue (OCT) samples was 15 minutes. Cylindrical OCTs were harvested from rabbit femoral condyles and then preserved for 1 week in the preservation solutions at 4 ℃. Then OCTs were reacted to bind bFGF-CBD for 15 minutes in the solution containing bFGF-CBD and then implanted to rabbit osteochondral defect models. While in the control samples, OCTs were not bound with bFGF-CBD. At 4 and 8 weeks after implantation, condyles were harvested and then samples were accessed using micro-CT and histological samples stained with HE and safranin-o. We could not observed significant difference between OCT samples with bFGF-CBD and control samples. These results concluded that bFGF-CBD might not improve osteochondral allografting of rabbit model.

Free Research Field

同種組織移植