2016 Fiscal Year Final Research Report
Basic research for the establishment of one-step cartilage repair technique using silk fibroin sponge with synovial cells and minced cartilage
| Project/Area Number |
26462322
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Toho University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 関節病学 / 組織工学 / 軟骨再生 |
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| Outline of Final Research Achievements |
[Purpose] To examine if human synovial cells can be differentiate into chondrocytes on silk fibroin sponges (FS). [Material and methods] Human synovial cells were seeded on FS and cultured in the conditioned media for chondrocyte differentiation in the presence or absence of BMP-2. The effect of co-culture with minced cartilage was investigated histologically and biochemically. [Results] Synovial cells cultured with BMP-2 were shown to differentiate into chondrocytes on FS. Expression of SOX9 and ACAN gene and COL-2/COL-1 expression rate were elevated by co-culture with minced cartilage. [Discussion] The results of this study suggest that FS with synovial cells and minced cartilage could apply to articular cartilage repair.
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| Free Research Field |
整形外科学
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