Effect of combined therapy with hydrogen gas and hypothermia in a rat model of transient forebrain ischemia

2016 Fiscal Year Final Research Report

• Project/Area Number: 26462328
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Anesthesiology
• Research Institution: Akita University
• Principal Investigator: Nagasaki Go 秋田大学, 医学部, 助教 (60292380)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 水素ガス / ラット / 前脳虚血モデル / 脳低温療法

Outline of Final Research Achievements

We examined whether combined treatment with 1.3% hydrogen gas and hypothermia reduces brain injury after transient forebrain ischemia in rats compared with either treatment alone. Rats were subjected to 10 minutes of forebrain ischemia followed by 7 days of reperfusion. Four groups were tested: group C (control); group G (hydrogen gas); group H (hypothermia, 34℃); and group GH (hydrogen gas + hypothermia). Neuronal damage in the CA1 subfield of the hippocampus was evaluated microscopically and graded on a damage score scale of 1,no damage～6, no intact neuron. The median injury score [interquartile range] was 5[0] for the group C, 4.5[2] for the group G, 3.5[1] for the group H and 2.5[2] for the group GH. The current study suggested that the neuroprotective efficacy of hypothermia could be increased by 1.3% hydrogen gas inhalation.

Free Research Field

麻酔科学