2016 Fiscal Year Final Research Report
Therapeutic strategy for reproductive aged women by regulation of oxidative stress and endoprasmic reticulum stress
| Project/Area Number |
26462474
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Yamagata University |
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Principal Investigator |
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| Research Collaborator |
MATSUO Koki 山形大学, 医学部, 助教 (90594317)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | マウス / 加齢卵 / 小胞体ストレス / salubrinal |
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| Outline of Final Research Achievements |
We examined whether poor embryo development of in vivo aged mouse oocytes (aged oocytes) could be improved by regulation of endoplasmic reticulum stress (Endoplasmic reticulum stress: ERS) or not. Because expression level of GRP78 (molecular chaperone induced by ERS) was the highest in unfertilized oocytes, we treated unfertilized aged oocytes with salburinal (inhibitor of ERS) before IVF. Salburinal treated aged oocytes showed significantly decrease in abnormal embryos and significantly increase in morula, blastocysts and hatched blastocysts. Regulation of ERS due to inhibition of dephosphorylation of eIF2α-PERK pathway by salburinal might improve embryo development of aged oocytes.
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| Free Research Field |
生殖医学
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