2016 Fiscal Year Final Research Report
Morphology and functional analysis of mitochondria in uterine leiomyoma
| Project/Area Number |
26462475
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Chiba University |
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Principal Investigator |
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| Research Collaborator |
KOBAYASHI Tatsuya
XU Linlin
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 子宮筋腫 / 低酸素 / HIF-1 / ミトコンドリア / 解糖 |
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| Outline of Final Research Achievements |
Severe hypoxia may cause mitochondrial dysfunction in uterine leiomyoma; therefore, we clarified hypoxic responses in uterine leiomyoma. Expression of hypoxia inducible factor (HIF)-1α was significantly higher in uterine leiomyoma tissues than those in the adjacent myometrium. Hypoxia significantly induced HIF-1α protein expression and intranuclear distribution of HIF-1α in primary cultured uterine leiomyoma cells. Hypoxia also induced recruitment of HIF-1αbinding to the putative HIF response element on the promoter of HIF-responseive genes and subsequent mRNA expression of the HIF-responsive genes in uterine leiomyoma cells. Hypoxia significantly induced apoptosis of uterine leiomyoma cells. Taken together, hypoxia may induce HIF-mediated hypoxic responses in uterine leiomyoma.
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| Free Research Field |
婦人科学
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