Study on the regeneration of gametes using parthenogenetic embryonic stem cells.

2016 Fiscal Year Final Research Report

• Project/Area Number: 26462481
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: University of Yamanashi
• Principal Investigator: HIRATA Shuji 山梨大学, 総合研究部, 教授 (00228785)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 単為発生 / 胚性幹細胞 / 雌性単為発生 / 雄性単為発生 / 初期胚 / ミトコンドリアの動態 / 生殖細胞の再生

Outline of Final Research Achievements

In this study, basic investigation of regeneration of gametes using parthenogenetic embryonic stem cells (pES) and androgenetic ES cells (aES cells). We established the aES cells, as well as pES cells, using microinjection of 2 sperm into the enucleated meta II oocyte. Sex chromosomal analysis on the aES cells revealed that all the aES cells have XX or XY karyotype, indicating that the X chromosome is essential for the early embryonic development. Moreover, to establish the high-quality ES cells for regeneration of gametes, we investigated the factors defining the early embryonic development. The results indicated that the intracellular dynamics of mitochondria in the one-cell embryo is one of the key factor of the early embryonic development.

Free Research Field

生殖医学