2016 Fiscal Year Final Research Report
The alterations of villous DNA methylation in first trimester associated with the pathogenesis of preeclampsia
| Project/Area Number |
26462501
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Showa University |
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Principal Investigator |
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| Research Collaborator |
Akihiro Kawashima 昭和大学, 医学部, 助教
Takenaka Shin 昭和大学, 医学部, 助教
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 妊娠高血圧症候群 / 妊娠絨毛 / DNAメチル化 |
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| Outline of Final Research Achievements |
We investigated placental DNA methylation in preeclampsia (PE) compared with normal case to understand epigenetic pathogenesis of PE. Placental DNA methylation profiles in 6 weeks, 10-11 weeks, normal cases at third trimester, and cases with PE were made, and they were compared among the groups. In the present study, we targeted CpG sites located on promoters. In comparison of DNA methylation profiles, 246 CpG sites on promoters were detected as the CpG sites that were differentially methylated in PE compared with normal third trimester. The cluster analysis for all samples was done using the 246 CpG sites. We confirmed that not only normal third trimester and PE, but also 6 weeks and 10-11 weeks were classified accurately.
DNA methylation pattern physiologically changes in the villous trophoblasts during the placentation. The insufficiency of placental formation and function leads to the onset of preeclampsia when the change is not sufficient in the villi in early gestation.
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| Free Research Field |
周産期学
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