2017 Fiscal Year Final Research Report
Study of the susceptibility to temporary ischemic insults in the vestibulo-cerebellum
| Project/Area Number |
26462547
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Gunma University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Keywords | patch-clamp / vestibulo-cerebellum / Purkinje cell |
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| Outline of Final Research Achievements |
To clarify the neural pathophysiology underlying central vertigo induced by transient ischemia, such as is observed in vertebrobasilar insufficiency, we focused on the excitatory circuits in the rat cerebellum. In our study, we investigated the effects of oxygen-glucose deprivation (OGD) on sEPSCs in Purkinje cells in vestibulo-cerebellar lobules IX and X. sEPSCs in Purkinje cells in lobules IX and X showed marked enhancement of frequency during the short term treatment of oxygen-glucose deprivation. On the other hand, OGD-induced enhancement of sEPSC was small in other control lobules IV-VI. Furthermore, the complete blockade of OGD-induced enhancement of sEPSCs by TTX indicates that this effect depend on spontaneous firings of presynaptic neurons. These results suggest that Purkinje cells in lobules IX and X are richer in the fundamental excitatory inputs than those in other vermal lobules, which results in high sensitivity to ischemic insults in vestibule-cerebellum.
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| Free Research Field |
神経耳科
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