Investigation of autophagy-regulated mechanism of vascular cell death in early diabetic retinopathy

2016 Fiscal Year Final Research Report

• Project/Area Number: 26462671
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: St. Marianna University School of Medicine
• Principal Investigator: Takagi Hitoshi 聖マリアンナ医科大学, 医学部, 教授 (70283596)
• Co-Investigator(Renkei-kenkyūsha): TAKAKURA Nobuyuki 大阪大学, 微生物学研究所, 教授 (80291954) ; KOYA Daisuke 金沢医科大学, 医学部, 教授 (70242980)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: オートファジー / 糖尿病網膜症 / 網膜血管細胞死

Outline of Final Research Achievements

Vascular cell death causes diabetic retinopathy and autophagy has been paid attention as a novel cell death mechanism. We compared gene expression between low and high glucose - stimulated vascular endothelial cells. High glucose-stimulation induced inflammatory and thrombotic effects in gene expression profile in vascular cells. TGFB2 specially upregulated and mediated cell death. High glucose condition upregulated autophagy and inhibited TNF-induced P62 increase, which are reversed by inhibition of autophagy reflux. These finding suggests that autophagy regulation could be applied to future treatment strategies.

Free Research Field

眼科学