2016 Fiscal Year Final Research Report
Analaysys of tumor-associated genes in human pediatric liver tumor and renal tumor using mice at each developmental stage
| Project/Area Number |
26462717
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pediatric surgery
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| Research Institution | Nihon University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 小児腫瘍学 / 肝芽腫 / 腎芽腫 / 機能解析 |
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| Outline of Final Research Achievements |
To newly identify genes involved in the development of hepatblastoma and neproblastoma, we analyzed the methylation status of the candidate genes in liver and kidney of mice at different developmental stages. In this analysis, we found that methylation levels of ZSCAN10、SlC16A5 were significantly higher in liver of elder mice. However, there was no significant difference in the expression levels of these genes between hepatoblastoma cells and liver tissues, and between neproblastoma and normal kidney tissues. In addition, no aberrant expression of both genes was observed in the surgical specimens of hepatoblastoma and neproblastoma. We then conducted the functional analysis of TFAP2E in heaptoblastoma. It this analysis TFAP2E silenced hepatoblastoma cells showed higher viability compared to that of control cells. On the other hand, forced expression of TFAP2E resulted in reduced cell viability. These results indicate that TFAP2E has a tumor suppressive function in hepatoblastoma.
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| Free Research Field |
小児外科学
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