2017 Fiscal Year Final Research Report
Evaluation of wound healing ability in patients with diabetes based on clonal conversion of epidermal keratinocyte stem cells
| Project/Area Number |
26462739
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Plastic surgery
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| Research Institution | Keio University (2015-2017)
St. Marianna University School of Medicine (2014) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
難波 大輔 東京医科歯科大学, 難治疾患研究所, 准教授 (10380255)
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| Research Collaborator |
Miyamoto Akira
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Keywords | 創傷治癒 / 糖尿病 / 重症下肢虚血 / 表皮角化幹細胞 / クローナル・コンバージョン / 細胞増殖 / 細胞分化 / 細胞遊走 |
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| Outline of Final Research Achievements |
Wound healing becomes prolonged in patients with diabetes. Healing is delayed even longer in patients with progressive diabetes who have developed chronic renal failure and peripheral arterial disease in the lower extremities (critical limb ischemia: CLI) as a complication of hemodialysis. This study focused on the relationship between epithelialization and the prolongation of wound healing in CLI. We evaluated cell proliferation by measuring the colony forming ability of epidermal keratinocytes from the trunks and feet of CLI patients. We also observed the migratory capacity of epidermal keratinocytes over time using a live imaging system. We found no clear difference in cell proliferation and migratory capacity between epidermal keratinocytes from CLI and from the trunk. Our findings suggest it is unlikely that epidermal keratinocyte stem cells from CLI result in significantly greater clonal conversion than trunk cells.
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| Free Research Field |
形成外科
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