2016 Fiscal Year Final Research Report
Mechanism of severe sepsis associated with hospital acquired infection
| Project/Area Number |
26462747
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Emergency medicine
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Research Collaborator |
HIRUMA Takahiro 東京大学, 医学部附属病院, 助教 (40572277)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 敗血症 / 急性腎障害 / 免疫抑制 |
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| Outline of Final Research Achievements |
We evaluate therapeutic possibility of IFNb on murine model of septic pneumonia. Murine sepsis was induced by cecal ligation and puncture (CLP) on mice. Four days later, pneumonia was made with intratracheal instillation of Pseudomonas aeruginosa. IFNb was given 24 hours before PA instillation. Survival was observed. Bronchoalveolar lavage (BAL) was done at 18 hours after PA instillation and remaining bacterial count, cell count, cytokine levels in the BALF were evaluated. Survival was significantly worse on murine model of septic pneumonia. Administration of IFNb significantly improved survival. TNFa and IL-6 in the BALF tended to be higher and remaining bacterial count tended to be lower in group treated by IFNb.Impaired host defense mechanism might associate with higher mortality in septic pneumonia mouse model. Subcutaneous IFNb may restore production of inflammatory cytokines in the lung and increased neutrophil recruitment and that may contribute to improve survival.
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| Free Research Field |
救急
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